FLT3 (D835V) Kinase Assay Service

Activity-based FLT3 (D835V) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
FLT3 (D835V)
Enzyme
Human FLT3 (D835V)
HGNC
FLT3
Synonym
FLK2, STK1, CD135
Substrates
Peptide substrate, [EAIYAAPFAKKK], 20 µM
ATP, 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 0.47
PP2 201
D-64406 15.1
Scientific Information
P36888 (http://www.uniprot.org/uniprot/P36888)
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound inhibition of FLT3 (D835V)
Reference compound IC50 for FLT3 (D835V)
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