FLT3 (F691L/D835Y) Kinase Assay Service

Activity-based FLT3 (F691L/D835Y) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.

Target Overview

CAT#

FLT3 (F691L/D835Y)

Enzyme

Human FLT3 (F691L/D835Y)

HGNC

FLT3 (F691L/D835Y)

Synonym

FL cytokine receptor [Precursor], Tyrosine-protein kinase receptor FLT3, Stem cell tyrosine kinase 1, STK-1, Flk-2

Scientific Information

P36888 (http://www.uniprot.org/uniprot/P36888)

Assay Properties

Substrates

Peptide substrate, [EAIYAAPFAKKK], 20 µM
ATP, 10 µM

Reaction

Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 0.4
PP2 >100,000
AG1478 2,800
EGFR/Erb Inhibitor >100,000
D-64406 220
Screening Location

Malvern, PA, USA

Further Information

More information can be found on our website Kinase Assays

Reference compound IC50 for FLT3 (F691L/D835Y)