FLT3 (ITD)-NPOS Kinase Assay Service

Activity-based FLT3 (ITD)-NPOS kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
FLT3 (ITD)-NPOS
Enzyme
Human FLT3 (ITD) (L610_E611insCSSDNEYFYVDFREYEYDLKWEFPRENL)
HGNC
FLT3 (ITD)-NPOS
Synonym
FLK2, STK1, CD135
Substrates
Peptide substrate, [EAIYAAPFAKKK], 20 µM
ATP, 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine4.1
PP210,000
AG1478730
PDGFR I-III310
D-6440655
Scientific Information
P36888 (http://www.uniprot.org/uniprot/P36888)
Screening Location
Malvern, USA
Reference compound IC50 for FLT3 (ITD)-NPOS
Reference compound IC50 for FLT3 (ITD)-NPOS