FLT3 (ITD)-W51 Kinase Assay Service

Activity-based FLT3 (ITD)-W51 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
FLT3 (ITD)-W51

Enzyme
Human FLT3 (ITD) (L601_K602insREYEYDL)

HGNC
FLT3 (ITD)-W51

Synonym
FLK2, STK1, CD135

Substrates
Peptide substrate, [EAIYAAPFAKKK], 20 µM
ATP, 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 1.2
PP2 3,600
AG1478 250
PDGFR I-III 94
D-64406 32
Scientific Information
P36888 (http://www.uniprot.org/uniprot/P36888)

Screening Location
Malvern, USA

Reference compound IC50 for FLT3 (ITD)-W51
Reference compound IC50 for FLT3 (ITD)-W51

PDF