Activity-based FLT3 ITD kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
FLT3 ITD
Enzyme
Human FLT3 ITD, mutant
HGNC
FLT3 (ITD)
Manning ID
FLT3
Synonym
FLT3, CD135, FLK2, STK-1, STK1, FLT3 ITD, FLT3 (ITD)
UniProt
P36888 (FLT3_HUMAN)
Kinase Type
Protein kinase
Kinase Group
TK
Assay Properties
Assay Format
33PanQinaseTM
Substrates
Poly(Ala,Glu,Lys,Tyr)6:2:5:1, ATP
Reaction
Poly(Ala,Glu,Lys,Tyr)6:2:5:1 + 33P-ATP = 33P-Poly(Ala,Glu,Lys,Tyr)6:2:5:1 + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 2 |
| Sorafenib | 520 |
| Sunitinb | 170 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | FLT3 ITD |
| 345 wt Profiler | No |
| 345 IC50 wt Profiler | No |
| Mutant Profiler Addon | Yes |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein |
Yes; ProQinaseTM – FLT3 ITD |
| Product Number | 0778-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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