FLT3 Kinase Activity Assay Service

Activity-based FLT3 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology

Target Overview

RBE Target ID

FLT3

Enzyme

Human FLT3, wt

HGNC

FLT3

Manning ID

FLT3

Synonym

FLK2, STK1, CD135, FLT3, STK-1, FLT3 wt

UniProt

P36888 (FLT3_HUMAN)

Kinase Type

Protein kinase

Kinase Group

TK

Assay Properties

Assay Format

33PanQinaseTM

Substrates

Poly(Ala,Glu,Lys,Tyr)6:2:5:1, ATP

Reaction

Poly(Ala,Glu,Lys,Tyr)6:2:5:1 + 33P-ATP = 33P-Poly(Ala,Glu,Lys,Tyr)6:2:5:1 + ADP

Readout

Enzymatic activity

Technical Reference Compound IC50 value(s)
Compounds IC50 (nM)
Staurosporine 1
Sorafenib 110
Sunitinb 18

Screening Location

Freiburg, Germany

Further Information

More information can be found on our website Kinase Assays

Service and Product Availability

Screening Opportunities
FreeChoice FLT3
345 wt Profiler Yes
345 IC50 wt Profiler Yes
Mutant Profiler Addon No
Lipid Kinase Profiler Addon No
IC50 Profiler No
Recombinant Protein Availability
Active recombinant protein

Yes; ProQinaseTM – FLT3
(Activity verified by 33PanQinaseTM-Assay)

Product Number 0776-0000-1
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