FLT3 (Y591_V592insVDFREYEYD) Kinase Assay Service

Activity-based FLT3 (Y591_V592insVDFREYEYD) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
FLT3 (Y591_V592insVDFREYEYD)
Enzyme
Human FLT3 (Y591_V592insVDFREYEYD)
HGNC
FLT3 (Y591_V592insVDFREYEYD)
Synonym
FLK2, STK1, CD135
Substrates
Peptide substrate, [EAIYAAPFAKKK], 20 µM
ATP, 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine0.9
PP22,300
AG1478190
PDGFR I-III150
D-6440618
Scientific Information
P36888 (http://www.uniprot.org/uniprot/P36888)
Screening Location
Malvern, USA
Reference compound IC50 for FLT3 (Y591_V592insVDFREYEYD)
Reference compound IC50 for FLT3 (Y591_V592insVDFREYEYD)