FMS Kinase Assay Service

Activity-based FMS kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
FMS
Enzyme
Human FMS
HGNC
CSF1R
Synonym
C-FMS, CD115, CSF-1-R, CSFR, FIM2, FMS
Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine1.7
PP22,200
AG1478>20,000
PDGFR I-III1,900
D-644067,400
Scientific Information
P07333 (http://www.uniprot.org/uniprot/P07333)
Screening Location
Malvern, USA
Reference compound IC50 for FMS
Reference compound IC50 for FMS