Activity-based FYN-A kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
FYN-A
Enzyme
Human FYN Isoform A
HGNC
FYN
Synonym
SLK, SYN, p59-Fyn, c-Fyn
Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP, 10 µM
ATP, 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 |
|---|---|
| Staurosporine | 4.5 nM |
| PP2 | 6.4 nM |
| AG1478 | 2.3 µM |
| D-64406 | 687 nM |
Scientific Information
P06241 (http://www.uniprot.org/uniprot/P06241)
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound inhibition of FYN-A
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