Activity-based FYN kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
FYN
Enzyme
Human FYN, wt
HGNC
FYN
Manning ID
FYN
Synonym
MGC45350, SLK, SYN, p59-FYN, cFYN, FYN, p59-Fyn, FYN wt
UniProt
P06241 (FYN_HUMAN)
Kinase Type
Protein kinase
Kinase Group
TK
Assay Properties
Assay Format
33PanQinaseTM
Substrates
PolyEY, ATP
Reaction
PolyEY + 33P-ATP = 33P-PolyEY + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 43 |
| Sorafenib | 9700 |
| Sunitinb | 660 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | FYN |
| 345 wt Profiler | Yes |
| 345 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein |
Yes; ProQinaseTM – FYN |
| Product Number | 0352-0000-1 |
| Web Shop | Reaction Biology Online Shop |
PDF