FYN (Y531F) Kinase Assay Service

Activity-based FYN (Y531F) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
FYN (Y531F)

Enzyme
Human FYN (Y531F)

HGNC
FYN (Y531F)

Synonym
SLK, SYN, p59-Fyn

Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 5.4
PP2 44
AG1478 1,100
PDGFR I-III >100,000
D-64406 690
Scientific Information
P06241 (http://www.uniprot.org/uniprot/P06241)

Screening Location
Malvern, USA

Reference compound IC50 for FYN (Y531F)
Reference compound IC50 for FYN (Y531F)

PDF