Activity-based G9a Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.
Histone-lysine N-methyltransferase EHMT2, Histone H3-K9 methyltransferase 3, Euchromatic histone-lysine N-methyltransferase 2, HLA-B-associated transcript 8 Protein G9a, Lysine N-methyltransferase 1C, EHMT2, BAT8, C6orf30, G9A, KMT1C, NG36
Histone H3, or H3 (1-21) peptide, 5 µM
SAM, 1 µM
S-adenosyl-L-[methyl-³H]methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N⁶-[methyl-³H]-L-lysine
|Compounds||IC50 (nM) With Histone H3 substrate|
|Compounds||IC50 (nM) With H3 (1-21) peptide substrate|
Malvern, PA, USA
More information can be found on our website Methyltransferase Assays.
Reference compound IC50 for G9a/Histone H3
Reference Compound IC50 for G9a/H3 (1-21)