G9a Methyltransferase Assay Service

Activity-based G9a Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.
Target Overview
CAT#
G9a
Enzyme
Human G9a
Synonym
Histone-lysine N-methyltransferase EHMT2, Histone H3-K9 methyltransferase 3, Euchromatic histone-lysine N-methyltransferase 2, HLA-B-associated transcript 8 Protein G9a, Lysine N-methyltransferase 1C, EHMT2, BAT8, C6orf30, G9A, KMT1C, NG36
Substrates
Histone H3, or H3 (1-21) peptide, 5 µMSAM, 1 µM
Assay Properties
Reaction
S-adenosyl-L-[methyl-³H]methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N⁶-[methyl-³H]-L-lysine
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM) With Histone H3 substrate
SAH2,300
Sinefungin94,000
BIX 01294>200,000
Chaetocin6,000
CompoundsIC50 (nM) With H3 (1-21) peptide substrate
SAH760
Sinefungin8,300
BIX 012945,300
Chaetocin8,000
Scientific Information
Q96KQ7 (http://www.uniprot.org/uniprot/Q96KQ7)
Screening Location
Malvern, USA
Reference compound IC50 for G9a/Histone H3
Reference compound IC50 for G9a/Histone H3
Reference Compound IC50 for G9a/H3 (1-21)
Reference Compound IC50 for G9a/H3 (1-21)