Activity-based GAK kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
GAK
Enzyme
Human GAK
HGNC
GAK
Synonym
DNAJC26
Scientific Information
O14976 (http://www.uniprot.org/uniprot/O14976)
Assay Properties
Substrates
Histone H3.3/H4 Tetramer Protein, 20 µM
ATP, 10 µM
ATP, 10 µM
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| LP-935509 | 135 |
| Staurosporine | 86 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound inhibition of GAK
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