Activity-based GCK/MAP4K2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
GCK
Enzyme
Human GCK
HGNC
MAP4K2
Synonym
BL44, GCK, RAB8IP
Substrates
MBP (Myelin Basic Protein), 10 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 1.9 |
| Ro 31-8220 | 886 |
| GW 5074 | 2,800 |
| H-89 | 22,500 |
Scientific Information
Q12851 (http://www.uniprot.org/uniprot/Q12851)
Screening Location
Malvern, USA
Reference compound IC50 for GCK
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