GLK/MAP4K3 Kinase Assay Service

Activity-based GLK/MAP4K3 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
GLK/MAP4K3

Enzyme
Human GLK/MAP4K3

HGNC
MAP4K3

Synonym
GLK

Substrates
MBP (Myelin Basic Protein), 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine <0.5
Ro 31-8220 160
GW 5074 7,200
H-89 9,600
Rottlerin 8,900
Scientific Information
Q8IVH8 (http://www.uniprot.org/uniprot/Q8IVH8)

Screening Location
Malvern, USA

Reference compound IC50 for GLK/MAP4K3
Reference compound IC50 for GLK/MAP4K3

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