GLP Methyltransferase Assay Service

Activity-based GLP Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.
Target Overview
CAT#
GLP
Enzyme
Human GLP
Synonym
Histone-lysine N-methyltransferase EHMT1, Euchromatic histone-lysine N-methyltransferase 1, G9a-like protein 1, EUHMTASE1, GLP, KIAA1876, KMT1D
Substrates
Histone H3, 5 µMSAM, 1 µM
Assay Properties
Reaction
S-adenosyl-L-[methyl-³H]methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N⁶-[methyl-³H]-L-lysine
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
SAH350
Sinefungin13,000
BIX 0129474,000
Chaetocin9,300
Scientific Information
Q9H9B1 (http://www.uniprot.org/uniprot/Q9H9B1)
Screening Location
Malvern, USA
Reference compound IC50 for GLP
Reference compound IC50 for GLP