GPBAR1 cAMP Agonist Assay for compound screening and profiling.
Target Information
Cell Catalog#
LLC-2049
GPCR Name
Human G-protein coupled bile acid receptor 1
Gene Name
GPBAR1
GPCR Sub Family
Bile acid receptors
Coupling Partner
Gαs
Uniprot Number
Q8TDU6 (https://www.uniprot.org/uniprotkb/Q8TDU6/entry)
Cell Type
U2OS/GPBAR1/β-arrestin-2 stable cell line
Assay Information
Assay Principle
The cAMP agonist assay detects agonist-induced inhibition of cAMP production via GPBAR1 activation in U2OS/GPBAR1/β-arrestin-2 stable cell line.
Assay Type
Cell-based functional assay
Functional Mode
Agonist
Assay Readout
FRET fluorescence
Reference Compound EC50
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website GPCR Assay and Profiling Services
Representative dose–response curve of reference agonist for GPBAR1
