GPBAR1 Functional Cell Reporter Agonist Assay for compound screening and profiling via luminescence recordings.
Target Overview
CAT#
GPBAR1 Functional Agonist
GPCR Target
Human GPBAR1
Cell Line
Proprietary human cells engineered to constitutively express GPBAR1
Scientific Information
GPBAR (https://www.uniprot.org/uniprotkb/Q8TDU6/entry)
Assay Properties
Protocol
Cells expressing GPBAR1 incubated with agonist for 24 h; luminescence measurement 30 min post application of detection reagent
Readout
Luminescence tracking changes in NFAT activity on Envision plate reader
Reference Compound EC50
| Compound | EC50 (nM) |
|---|---|
| Lithocholic Acid (LCA) | 1124 |
| TC-G 1005 | 0.34 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website GPCR Assay and Profiling Services
Reference compound activation of GPBAR1
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