Granzyme B Protease Assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
Granzyme B
Enzyme
Human Granzyme B
Synonym
Granzyme-2, C11, CTLA-1, Cathepsin G-like 1, Cytotoxic T-lymphocyte proteinase 2, Fragmentin-2
Substrates
Fluorogenic substrate Z-IETD-AFC, 50 µM
Assay Properties
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (µM) |
| IETD-CHO | 0.18 |
Scientific Information
P10144 (http://www.uniprot.org/uniprot/P10144)
Screening Location
Malvern, USA
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