GRK2 Kinase Assay Service

Activity-based GRK2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
GRK2
Enzyme
Human GRK2
HGNC
GRK2
Synonym
ADRBK1
Substrates
Casein, 1 mg/ml
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine802
Ro 31-822017,400
H-8910,800
Scientific Information
P25098 (http://www.uniprot.org/uniprot/P25098)
Screening Location
Malvern, USA
Reference compound IC50 for GRK2
Reference compound IC50 for GRK2