Activity-based GRK3 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
GRK3
Enzyme
Human GRK3
HGNC
GRK3
Synonym
ADRBK2
Substrates
Casein, 1 mg/ml
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 680 |
| Ro 31-8220 | 83,000 |
| H-89 | 11,000 |
Scientific Information
P35626 (http://www.uniprot.org/uniprot/P35626)
Screening Location
Malvern, USA
Reference compound IC50 for GRK3
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