GRK4 Kinase Assay Service

Activity-based GRK4 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
GRK4

Enzyme
Human GRK4

HGNC
GRK4

Synonym
GPRK2L, GPRK4, GRK4a, IT11

Substrates
Casein, 1 mg/ml
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 172
Ro 31-8220 1,300
H-89 38,000
Scientific Information
P32298 (http://www.uniprot.org/uniprot/P32298)

Screening Location
Malvern, USA

Reference compound IC50 for GRK4
Reference compound IC50 for GRK4

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