GSK3b Kinase Assay Service

Activity-based GSK3b kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
GSK3b
Enzyme
Human GSK3b
HGNC
GSK3B
Synonym
GSK3beta
Substrates
Peptide substrate, [YRRAAVPPSPSLSRHSSPHQ(pS)EDEEE], 20 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine4.2
Ro 31-82201.8
GW 50743,500
H-89>100,000
Rottlerin37,400
Scientific Information
P49841 (http://www.uniprot.org/uniprot/P49841)
Screening Location
Malvern, USA
Reference compound IC50 for GSK3b
Reference compound IC50 for GSK3b