GSK3b Kinase Assay Service

Activity-based GSK3b kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
GSK3b

Enzyme
Human GSK3b

HGNC
GSK3B

Synonym
GSK3beta

Substrates
Peptide substrate, [YRRAAVPPSPSLSRHSSPHQ(pS)EDEEE], 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 4.2
Ro 31-8220 1.8
GW 5074 3,500
H-89 >100,000
Rottlerin 37,400
Scientific Information
P49841 (http://www.uniprot.org/uniprot/P49841)

Screening Location
Malvern, USA

Reference compound IC50 for GSK3b
Reference compound IC50 for GSK3b

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