H1 Functional Cell-based Antagonist Assay for compound screening and profiling via fluorescence-based quantification of intracellular calcium.
Target Overview
CAT#
H1 Functional Antagonist
GPCR Target
Human H1
Cell Line
HEK293/H1
Scientific Information
HRH1 (https://www.uniprot.org/uniprotkb/P35367/entry)
Assay Properties
Reaction
30 min incubation with antagonist; H1 activation by agonist application using FLIPR Penta system cells treated with Calcium 6 dye; fluorescence kinetic measurement
Readout
Calcium 6 dye fluorescence tracking changes in intracellular calcium
Reference Compound IC50
| Compound | IC50 (nM) |
|---|---|
| Cetirizine | 664 |
| Mepyramine | 16.2 |
| Fexofenadine | 1281 |
| Agonist | (nM) |
|---|---|
| Histamine | 100 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website GPCR Assay and Profiling Services
Reference compound inhibition of H1
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