Activity-based Haspin kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
Haspin/GSG2
Enzyme
Human Haspin/GSG2
HGNC
GSG2
Synonym
GSG2
Substrates
Histone H3, 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 12.3 |
| Ro 31-8220 | 8,700 |
| GW 5074 | 25.8 |
| H-89 | 301 |
| Rottlerin | 30,000 |
Scientific Information
Q8TF76 (http://www.uniprot.org/uniprot/Q8TF76)
Screening Location
Malvern, USA
Reference compound IC50 for Haspin/GSG2
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