HCK Kinase Assay Service

Activity-based HCK kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
HCK
Enzyme
Human HCK
HGNC
HCK
Synonym
JTK9
Substrates
Peptide substrate, [KVEKIGEGTYGVVYK], 20 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine1.7
PP246
AG1478540
D-644061,400
Scientific Information
P08631 (http://www.uniprot.org/uniprot/P08631)
Screening Location
Malvern, USA
Reference compound IC50 for HCK
Reference compound IC50 for HCK