HCK Kinase Assay Service

Activity-based HCK kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
HCK

Enzyme
Human HCK

HGNC
HCK

Synonym
JTK9

Substrates
Peptide substrate, [KVEKIGEGTYGVVYK], 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 1.7
PP2 46
AG1478 540
D-64406 1,400
Scientific Information
P08631 (http://www.uniprot.org/uniprot/P08631)

Screening Location
Malvern, USA

Reference compound IC50 for HCK
Reference compound IC50 for HCK

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