hERG Cardiac Safety Assay Service

Biochemical hERG Assay to assess cardiac safety of new compounds using fluorescence polarization for measuring compound binding to hERG.

Target Overview

CAT#:

hERG

Enzyme

Human ERG

Probe:

Predictor Tracer, 1 nM (ThermoFisher)

Assay Properties

Readout

Competition binding assay with Tracer

Reference Compound IC50s:
Compounds IC50 (nM)
E-4031 7.2
Astemizole 0.84
GBR 12909 21.5

 

Scientific Information:

P07900 (http://www.uniprot.org/uniprot/P07900)

IC50 determination hERG binding

PDF