Activity-based HUNK kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
HUNK
Enzyme
Human HUNK
HGNC
HUNK
Synonym
MAKV, B19
Substrates
MBP (Myelin Basic Protein), 20 µM
ATP, 10 µM
ATP, 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 |
|---|---|
| Staurosporine | 27 nM |
| GW 5074 | 310.1 nM |
| H-89 | >100 µM |
Scientific Information
P57058 (http://www.uniprot.org/uniprot/P57058)
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound inhibition of HUNK
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