IKKa/CHUK Kinase Assay Service

Activity-based IKKa/CHUK kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
IKKa/CHUK
Enzyme
Human IKKa/CHUK
HGNC
CHUK
Synonym
IKBKA, IKK1, IKKA, NFKBIKA, TCF16, IKK-alpha
Substrates
Peptide substrate, [KKKKERLLDDRHDSGLDSMKDEE], 20 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine280
Ro 31-822020,000
GW5074>20,000
Rottlerin4,200
Scientific Information
O15111 (http://www.uniprot.org/uniprot/O15111)
Screening Location
Malvern, USA
Reference compound IC50 for IKKa/CHUK
Reference compound IC50 for IKKa/CHUK