Activity-based IKKalpha kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
IKKalpha
Enzyme
Human IKKalpha, wt
HGNC
CHUK
Manning ID
IKKa
Synonym
IKK-alpha, IKBKA, IKK1, IKKA, NFKBIKA, TCF16, CHUK, IKKalpha, IKKa/CHUK
UniProt
O15111 (IKKA_HUMAN)
Kinase Type
Protein kinase
Kinase Group
Other
Assay Properties
Assay Format
33PanQinaseTM
Substrates
RBER-CHKtide, ATP
Reaction
RBER-CHKtide + 33P-ATP = 33P-RBER-CHKtide + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 123 |
| Sorafenib | 10000 |
| Sunitinb | 17000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | IKKalpha |
| 345 wt Profiler | Yes |
| 345 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein | No |
| Product Number | N/A |
| Web Shop | Reaction Biology Online Shop |
PDF