IKKb/IKBKB Kinase Assay Service

Activity-based IKKb/IKBKB kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
IKKb/IKBKB
Enzyme
Human IKKb/IKBKB
HGNC
IKBKB
Synonym
IKK2, IKKB, NFKBIKB, IKK-beta
Substrates
Peptide substrate, [KKKKERLLDDRHDSGLDSMKDEE], 20 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine405
Scientific Information
O14920 (http://www.uniprot.org/uniprot/O14920)
Screening Location
Malvern, USA
Reference compound IC50 for IKKb/IKBKB
Reference compound IC50 for IKKb/IKBKB