IKKbeta Kinase Activity Assay Service

Activity-based IKKbeta kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology

Target Overview

RBE Target ID

IKKbeta

Enzyme

Human IKKbeta, wt

HGNC

IKBKB

Manning ID

IKKb

Synonym

IKK-beta, IKK2, IKKB, NFKBIKB, IKBKB, IKKbeta, IKKb/IKBKB

UniProt

O14920 (IKKB_HUMAN)

Kinase Type

Protein kinase

Kinase Group

Other

Assay Properties

Assay Format

33PanQinaseTM

Substrates

RBER-CHKtide, ATP

Reaction

RBER-CHKtide + 33P-ATP = 33P-RBER-CHKtide + ADP

Readout

Enzymatic activity

Technical Reference Compound IC50 value(s)
Compounds IC50 (nM)
Staurosporine 320
Sorafenib 10000
Sunitinb 60000

Screening Location

Freiburg, Germany

Further Information

More information can be found on our website Kinase Assays

Service and Product Availability

Screening Opportunities
FreeChoice IKKbeta
345 wt Profiler Yes
345 IC50 wt Profiler Yes
Mutant Profiler Addon No
Lipid Kinase Profiler Addon No
IC50 Profiler No
Recombinant Protein Availability
Active recombinant protein

Yes; ProQinaseTM – IKKbeta
(Activity verified by 33PanQinaseTM-Assay)

Product Number 0258-0000-1
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