IKKe/IKBKE Kinase Assay Service

Activity-based IKKe/IKBKE kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
IKKe

Enzyme
Human IKKe

HGNC
IKBKE

Synonym
IKKI, IKK-i, KIAA0151, IKK-epsilon

Substrates
Casein, 2 mg/ml
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 0.3
Ro 31-8220 3,200
GW 5074 72,000
Scientific Information
Q14164 (http://www.uniprot.org/uniprot/Q14164)

Screening Location
Malvern, USA

Reference compound IC50 for IKKepsilon/IKBKE
Reference compound IC50 for IKKepsilon/IKBKE

PDF