Activity-based IRAK2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
IRAK2
Enzyme
Human IRAK2
HGNC
IRAK2
Synonym
Interleukin-1 receptor-associated kinase-like 2
Substrates
Peptide substrate, [modified-CGRTGRRNSI-NH2], 20 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 2.8 |
| Ro 31-8220 | 670 |
| GW5074 | >100,000 |
| H-89 | 108 |
| Rottlerin | >100,000 |
Scientific Information
O43187 (http://www.uniprot.org/uniprot/O43187)
Screening Location
Malvern, USA
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