Activity-based IRAK3 kinase assay for compound screening and profiling via Probe-Displacement kinase assay.
Target Overview
CAT#
IRAK3 (Binding)
Enzyme
Human IRAK3
HGNC
IRAK3
Synonym
IRAK-M
Assay Properties
Probe
Staurosporine-Red, 5 nM
Assay Format
Binding interaction between Staurosporine-Red and Kinase is quantified by HTRF technology using anti Tag HTRF reagents. Compounds binding in this binding pocket displace fluorescently labeled probe resulting loss of binding signal.
Readout
HTRF Readout, Probe-Displacement
Reference Compound IC50s
| Compounds | IC50 |
|---|---|
| Staurosporine | 0.3 nM |
| Dasatinib | 1.2 µM |
| PP2 | 266 nM |
| Sunitinib | 390 nM |
Scientific Information
Q9Y616 (http://www.uniprot.org/uniprot/Q9Y616)
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound inhibition of IRAK3
