IRAK3

NanoBRET Target Engagement Intracellular Kinase Assay, Competitive binding assay format

Target

IRAK3

HGNC Symbol

IRAK3

Synonyms

IRAK-M

Cell Line

HEK293 human epithelial cell line from ATCC

Assay Format

384-well plate format

Assay Protocol

HEK293 cells transiently expressing IRAK3-NanoLuc fusion vector were seeded into the wells of 384-well plates. The cells were pre-treated with the tracer 05 and then treated with reference compound CTx-0294885 and Crizotinib for 1 hour. NanoBRET signal was measured on an Envision 2104 Multilabel Reader. IC50 (EC50) value was calculated and IC50 curve was plotted using the GraphPad Prism 4 program based on a sigmoidal dose-response equation.

Readout

Binding of compound to target kinase

Reference Compound IC50s
Compounds IC50 (M)
CTx-0294885 6.001e-009
Crizotinib 2.536e-009
Screening Location

Malvern, USA

Reference compound IC50 for IRAK3