Activity-based IRAK4 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
IRAK4
Enzyme
Human IRAK4, wt
HGNC
IRAK4
Manning ID
IRAK4
Synonym
REN64, NY-REN-64, IRAK4
UniProt
Q9NWZ3 (IRAK4_HUMAN)
Kinase Type
Protein kinase
Kinase Group
TKL
Assay Properties
Assay Format
33PanQinaseTM
Substrates
RB-CTF, ATP
Reaction
RB-CTF + 33P-ATP = 33P-RB-CTF + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 50 |
| Sorafenib | 10000 |
| Sunitinb | 1600 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | IRAK4 |
| 345 wt Profiler | Yes |
| 345 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein |
Yes; ProQinaseTM – IRAK4 |
| Product Number | 1752-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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