IRAK4 NanoBRET Kinase Assay

IRAK4 NanoBRET Target Engagement Intracellular Kinase Assay for screening of compounds binding to the target.
Target Overview



HGNC Symbol




Cell Line

HEK293 human epithelial cell line from ATCC

Assay Properties

Assay Format

384-well plate format

Assay Protocol

HEK293 cells transiently expressing IRAK4-NanoLuc fusion vector were seeded into the wells of 384-well plates. The cells were pre-treated with the tracer 05 and then treated with reference compound CTx-0294885 for 1 hour. NanoBRET signal was measured on an Envision 2104 Multilabel Reader. IC50 (EC50) value was calculated and IC50 curve was plotted using the GraphPad Prism 4 program based on a sigmoidal doseresponse equation.


Binding of compound to target kinase

Reference Compound IC50s
Compounds IC50 (M)
CTx-0294885 1.489e-008

Screening Location

Malvern, USA

Reference compound IC50 for IRAK4