ITK Kinase Assay Service

Activity-based ITK kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
ITK

Enzyme
Human ITK

HGNC
ITK

Synonym
EMT 2, LYK 2, PSCTK2 2

Substrates
MBP (Myelin Basic Protein), 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 6.4
PP2 >20,000
AG1478 19,000
PDGFR I-III >20,000
D-64406 3,000
Scientific Information
Q08881 (http://www.uniprot.org/uniprot/Q08881)

Screening Location
Malvern, USA

Reference compound IC50 for ITK
Reference compound IC50 for ITK