JAK2 JH1&2 Kinase Assay Service

Activity-based JAK2 JH1&2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#

JAK2 JH1&2

Enzyme

Human JAK2 JH1&2

HGNC

JAK2 JH1&2

Synonym

Tyrosine-protein kinase JAK2, Janus kinase 2

Substrates

Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM

Assay Properties
Reaction

Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 1.1
PP2 >100,000
AG1478 >100,000
EGFR/ErbB Inhibitor >100,000
D-64406 22,000

 

Scientific Information
Screening Location

Malvern, USA

Reference compound IC50 for JAK2 JH1&2

reference compounds IC50 JAK2 JH1&2