Activity-based JAK2 JH1&2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
JAK2 JH1&2
Enzyme
Human JAK2 JH1&2
HGNC
JAK2 JH1&2
Synonym
Tyrosine-protein kinase JAK2, Janus kinase 2
Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 1.1 |
| PP2 | >100,000 |
| AG1478 | >100,000 |
| EGFR/ErbB Inhibitor | >100,000 |
| D-64406 | 22,000 |
Scientific Information
Screening Location
Malvern, USA
Reference compound IC50 for JAK2 JH1&2
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