Activity-based JAK3 JH1 & JH2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
JAK3 JH1 & JH2
Enzyme
Human JAK3 JH1 & JH2
HGNC
JAK3
Synonym
JAKL, L-JAK, LJAK
Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP, 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 2.8 |
| PP2 | 16.8 |
| AG1478 | 854 |
| D-64406 | 203 |
Scientific Information
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound inhibition of JAK3 JH1 & JH2
PDF