JAK3 Kinase Assay Service

Activity-based JAK3 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
JAK3

Enzyme
Human JAK3

HGNC
JAK3

Synonym
JAKL, L-JAK, LJAK

Substrates
Peptide substrate, [GGEEEEYFELVKKKK], 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine <0.1
D-64406 16,000
Scientific Information
P52333 (http://www.uniprot.org/uniprot/P52333)

Screening Location
Malvern, USA

Reference compound IC50 for JAK3
Reference compound IC50 for JAK3

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