JAK3 Kinase Assay Service

Activity-based JAK3 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
JAK3
Enzyme
Human JAK3
HGNC
JAK3
Synonym
JAKL, L-JAK, LJAK
Substrates
Peptide substrate, [GGEEEEYFELVKKKK], 20 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine<0.1
D-6440616,000
Scientific Information
P52333 (http://www.uniprot.org/uniprot/P52333)
Screening Location
Malvern, USA
Reference compound IC50 for JAK3
Reference compound IC50 for JAK3