Activity-based JNK1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
JNK1
Enzyme
Human JNK1
HGNC
MAPK8
Synonym
PRKM8, SAPK1, JNK1-alpha1
Substrates
ATF2, 3 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 2,400 |
| Ro 31-8220 | 18,000 |
| GW 5074 | 181,000 |
| Rottlerin | 15,000 |
Scientific Information
P45983-2 (http://www.uniprot.org/uniprot/P45983-2)
Screening Location
Malvern, USA
Reference compound IC50 for JNK1
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