Activity-based JNK2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
JNK2
Enzyme
Human JNK2
HGNC
MAPK9
Synonym
PRKM9, SAPK, p54a, JNK-alpha2, JNK2A, JNK2B
Substrates
ATF2, 3 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 4,000 |
| Ro 31-8220 | 13,000 |
| GW 5074 | 12,000 |
| Rottlerin | >20,000 |
Scientific Information
P45984-1 (http://www.uniprot.org/uniprot/P45984-1)
Screening Location
Malvern, USA
Reference compound IC50 for JNK2
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