JNK3 NanoBRET Target Engagement Intracellular Kinase Assay for screening of compounds binding to the target.
Target Overview
Target
JNK3
HGNC Symbol
MAPK10
Synonyms
MAPK10; JNK3A; PRKM10; p493F12
Cell Line
HEK293 human epithelial cell line from ATCC
Assay Properties
Assay Format
384-well plate format using Promega’s NanoBRET TE Intracellular Kinase Assay
Assay Protocol
HEK293 cells transiently expressing JNK3-NanoLuc Fusion Vector were seeded into the wells of 384-well plates. The cells were pre-treated with NanoBRET Tracer K-10 and then treated with reference compound JNK-IN-7 for 1 hour. The BRET signal was measured on an Envision 2104 Multilabel Reader. IC50 value was calculated and IC50 curve was plotted using the GraphPad Prism program based on a sigmoidal dose response equation.
Readout
Binding of compound to target
Reference Compound IC50s
| Compounds | IC50 (M) |
|---|---|
| JNK-IN-7 | 3.412e-009 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website NanoBRET Assay Services
Reference Compound IC50 for JNK3
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