KDR/VEGFR2 Kinase Assay Service

Activity-based KDR/VEGFR2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
KDR/VEGFR2

Enzyme
Human KDR/VEGFR2

HGNC
KDR

Synonym
VEGFR2, FLK1

Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 1.6
PP2 158
AG1478 184
PDGFR I-III 11,400
D-64406 651
Scientific Information
P35968 (http://www.uniprot.org/uniprot/P35968)

Screening Location
Malvern, USA

Reference compound IC50 for KDR/VEGFR2
Reference compound IC50 for KDR/VEGFR2

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