KIT D816H Kinase Activity Assay Service

Activity-based KIT D816H kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology

Target Overview

RBE Target ID

KIT D816H

Enzyme

Human KIT D816H, mutant

HGNC

KIT (D816E)

Manning ID

KIT

Synonym

KIT, C-Kit, PBT, CD117, SCFR, c-kit, KIT D816H, c-KIT (D816E)

UniProt

P10721 (KIT_HUMAN)

Kinase Type

Protein kinase

Kinase Group

TK

Assay Properties

Assay Format

33PanQinaseTM

Substrates

PolyEY, ATP

Reaction

PolyEY + 33P-ATP = 33P-PolyEY + ADP

Readout

Enzymatic activity

Technical Reference Compound IC50 value(s)
Compounds IC50 (nM)
Staurosporine 22
Sorafenib 3400
Sunitinb 940

Screening Location

Freiburg, Germany

Further Information

More information can be found on our website Kinase Assays

Service and Product Availability

Screening Opportunities
FreeChoice KIT D816H
345 wt Profiler No
345 IC50 wt Profiler No
Mutant Profiler Addon Yes
Lipid Kinase Profiler Addon No
IC50 Profiler IC50 KIT Profiler
Recombinant Protein Availability
Active recombinant protein

Yes; ProQinaseTM – KIT D816H
(Activity verified by 33PanQinaseTM-Assay)

Product Number 1041-0000-1
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