KIT V559D Kinase Activity Assay Service

Activity-based KIT V559D kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology

Target Overview

RBE Target ID

KIT V559D

Enzyme

Human KIT V559D, mutant

HGNC

KIT (V559D)

Manning ID

KIT

Synonym

KIT, C-Kit, PBT, CD117, SCFR, c-kit, KIT V559D, c-KIT (V559D)

UniProt

P10721 (KIT_HUMAN)

Kinase Type

Protein kinase

Kinase Group

TK

Assay Properties

Assay Format

33PanQinaseTM

Substrates

PolyEY, ATP

Reaction

PolyEY + 33P-ATP = 33P-PolyEY + ADP

Readout

Enzymatic activity

Technical Reference Compound IC50 value(s)
Compounds IC50 (nM)
Staurosporine 84
Sorafenib 420
Sunitinb 1500

Screening Location

Freiburg, Germany

Further Information

More information can be found on our website Kinase Assays

Service and Product Availability

Screening Opportunities
FreeChoice KIT V559D
345 wt Profiler No
345 IC50 wt Profiler No
Mutant Profiler Addon Yes
Lipid Kinase Profiler Addon No
IC50 Profiler IC50 KIT Profiler
Recombinant Protein Availability
Active recombinant protein

Yes; ProQinaseTM – KIT V559D
(Activity verified by 33PanQinaseTM-Assay)

Product Number 1047-0000-1
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