KIT (V559D,V654A) NanoBRET Kinase Assay

KIT (V559D,V654A) NanoBRET Target Engagement Intracellular Kinase Assay for screening of compounds binding to the target.

Target Overview


KIT (V559D,V654A)

HGNC Symbol

KIT (V559D/V654A)


C-Kit, PBT, CD117, SCFR

Cell Line

HEK293 human epithelial cell line from ATCC

Assay Properties

Assay Format

384-well plate format using Promega’s NanoBRET TE Intracellular Kinase Assay

Assay Protocol

HEK293 cells transiently expressing NanoLuc-KIT(V559D,V654A) Fusion Vector were seeded into the wells of 384-well plates. The cells were pre-treated with the NanoBRET Tracer K-4 and then treated with reference compound Dasatinib for 1 hour. The BRET signal was measured on an Envision 2104 Multilabel Reader. IC50 value was calculated and IC50 curve was plotted using the GraphPad Prism 4 program based on a sigmoidal dose response equation.


Binding of compound to target kinase

Reference Compound IC50s
Compounds IC50 (M)
Dasatinib 3.249e-008

Screening Location

Malvern, USA

Reference compound IC50 for KIT (V559D,V654A)