KOP Functional Cell-based Agonist Assay for compound screening and profiling via fluorescence-based quantification of intracellular calcium.
Target Overview
CAT#
KOP Functional Agonist
GPCR Target
Human KOP
Cell Line
CHO-K1/OPRK1/Gα15
Scientific Information
OPRK1 (https://www.uniprot.org/uniprotkb/P41145/entry)
Assay Properties
Protocol
KOP activation by agonist application using FLIPR Penta system cells treated with Calcium 6 dye; fluorescence kinetic measurement
Readout
Calcium 6 dye fluorescence tracking changes in intracellular calcium
Reference Compound EC50
| Compounds | EC50 (nM) |
|---|---|
| Asimadoline | 81 |
| U-69593 | 40 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website In Vitro Safety Pharmacology Profiling
Reference compound activation of KOP
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